发明名称 |
Method for detecting oligonucleotides and determining base sequence of nucleic acids |
摘要 |
<p>Disclosed are a method which can simultaneously analyze a plurality of analytes and can realize the determination of the base sequence of a large quantity of gene information in a short time, and an apparatus for the method. The method comprises the steps of: preparing four samples, for each of a plurality of nucleic acid analytes, containing analyte nucleic acid-derived oligonucleotide fragments with the end bases having been base-specifically fragmented; labeling the oligonucleotide fragments with a different label for each of the analyte nucleic acids; and subjecting the four samples to an analytical method which can distinguish oligonucleotide fragments based on a difference in length of one base, thereby determining the base sequence of the target nucleic acids. <IMAGE></p> |
申请公布号 |
EP0979878(A2) |
申请公布日期 |
2000.02.16 |
申请号 |
EP19990305648 |
申请日期 |
1999.07.16 |
申请人 |
THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH;HAYASHIZAKI, YOSHIHIDE |
发明人 |
HAYASHIZAKI, YOSHIHIDE |
分类号 |
G01N33/58;B01L3/00;C12N15/09;C12Q1/68;G01N27/447;(IPC1-7):C12Q1/68;B01D57/02;G01N21/64 |
主分类号 |
G01N33/58 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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