Cleavage of caulobacter produced recombinant fusion proteins

摘要

This invention provides a method for cleaving target proteins from Caulobacter S-layer protein under mild acid conditions. A fusion protein secreted by Caulobacter which includes a target protein and a Caulobacter S-layer secretion signal may be cleaved at an aspartate-proline dipeptide without solubilizing the fusion protein. This method may be carried out while the fusion protein is in an insoluble aggregate which facilitates recovery of the protein. This invention also provides a method of preparing a DNA construct for expression of the fusion protein and a method of preparing the fusion protein.