A SUBFAMILY OF RNA HELICASES WICH ARE MODULATORS OF THE FIDELITY OF TRANSLATION TERMINATION AND USES THEREOF

摘要

This invention provides a method of modulating translation termination efficiency of mRNA and/or promoting degradation of abberant transcripts. Als o, this invention provides a method of screening for a drug active involved in enhancing translation termination and a method for identifying a disease sta te involving defective the protein complex. This invention provides a purified complex comprising an amount of MTT1, human Upf1p, a peptidyl eucaryotic release factor 1 (eRF1) and a peptidyl eucaryotic release factor 3 (eRF3) effective to modulate translation termination. Further, this invention provides an expression vector which comprises a nucleic acid encoding operab ly linked to a regulatory element, as well as an antibody which binds to the complex. This invention provides an agent which inhibits or modulates the binding of MTT1 to eRF3. The agent may inhibit or facilitate the binding of MTT1 to eRF3.