发明名称 |
Liquid reagent for calcium assay |
摘要 |
<p>The present invention relates to an enzyme immunoassay method for assaying calcium ion, including putting calcium-dependent glutamate dehydrogenase, namely glutamate dehydrogenase (GLDH) never expressing its activity in the absence of calcium ion, in contact to the calcium ion in a sample to assay the enzyme activity of the GLDH, the activity changing depending on the calcium ion concentration in the sample; and the invention also relates to an assay reagent for use in the same. Because the reagent is very stable in a solution state under storage, the reagent can be incorporated in a liquid reagent. Thus, the calcium in a biological material or other samples can be assayed in a simple and accurate manner. <IMAGE></p> |
申请公布号 |
EP0976834(A2) |
申请公布日期 |
2000.02.02 |
申请号 |
EP19990114742 |
申请日期 |
1999.07.28 |
申请人 |
ORIENTAL YEAST CO., LTD. |
发明人 |
MATSUKAWA, HIROKAZU;OKA, OSAMU;FUJITA, TUYOSI;SAKAKIBARA, HITOSHI;SUGIYAMA, TATSUO |
分类号 |
C12N15/09;C12N1/21;C12N9/06;C12N9/08;C12N15/53;C12Q1/32;G01N33/84;(IPC1-7):C12Q1/32 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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