发明名称 |
Identification of eukaryotic growth-related genes and promoter isolation vector and method of use |
摘要 |
A polynucleotide encoding chitin synthase (CHS1), an enzyme essential for cell wall synthesis and yeast cell growth, is provided. A maltose responsive promoter (MRP) isolated using the promoter library of the invention is also described. The present invention also provides a vector for isolation of a eukaryotic regulatory polynucleotide, i.e., promoter. The vector is useful in the method of the invention which comprises identifying a eukaryotic regulatory polynucleotide, i.e., promoter region, by complementing the growth of an auxotrophic host cell containing the vector of the invention, which includes a promoter region operably linked to a promoterless auxotrophic gene. The vector is introduced into the host cell chromosome by targeted integration. Also provided is a library containing host cells having the vector of the invention integrated in the chromosome of the host cell.
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申请公布号 |
US6020133(A) |
申请公布日期 |
2000.02.01 |
申请号 |
US19980004225 |
申请日期 |
1998.01.08 |
申请人 |
MILLENNIUM PHARMACEUTICALS, INC. |
发明人 |
KOLTIN, YIGAL;RIGGLE, PERRY;GAVRIAS, VICKY;BULAWA, CHRIS;WINTER, KEN |
分类号 |
C12N15/09;C07K16/40;C12N1/15;C12N1/16;C12N1/21;C12N9/10;C12N15/00;C12N15/54;C12N15/67;C12N15/81;C12Q1/02;C12Q1/68;C12R1/725;(IPC1-7):C12Q1/68;C12Q1/48;A61K38/45 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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