| 摘要 |
The invention relates to a method for conducting the synthesis of nucleic acid molecules. The invention especially relates to a method which is carried out in a recursive manner. The nucleic acid constituents are preferably of a synthetic or semisynthetic origin. According to the inventive method, an additional nucleic acid molecule is attached to and/or coupled with a prepared nucleic acid molecule. The end of the prepared nucleic acid molecule is masked if no additional nucleic acid molecule is attached to or coupled with the same. The additional nucleic acid molecule is split at a predetermined point, resulting in an end to or with which an additional nucleic acid molecule can be attached and/or coupled. The aforementioned method steps can be repeated as often as required until the desired product is synthesized. The invention also relates to a kit for carrying out the inventive method. |
