发明名称 |
Purine nucleoside phosphorylase gene therapy for human malignancy |
摘要 |
The present invention provides a method of killing replicating or non-replicating, transfected or transduced mammalian cells and bystander cells, comprising: (a) transfecting or transducing mammalian cells with a nucleic acid encoding a non-human purine cleavage enzyme; and (b) contacting the transfected or transduced cells with an effective amount of a substrate for the purine cleavage enzyme, wherein the substrate is non-toxic to mammalian cells and is cleaved by the enzyme to yield a purine toxic to the targeted mammalian cells and bystander cells, to kill the mammalian cells expressing the enzyme and the bystander cells. Further provided is a vector comprising a DNA sequence coding for a non-human purine nucleoside phosphorylase protein and the vector is capable of replication and/or expression in a host which comprises, in operable linkage: a) optionally, an origin of replication; b) a promoter; and c) a DNA sequence coding for said protein.
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申请公布号 |
US6017896(A) |
申请公布日期 |
2000.01.25 |
申请号 |
US19970881772 |
申请日期 |
1997.06.24 |
申请人 |
UNIVERSITY OF ALABAMA RESEARCH FOUNDATION AND SOUTHERN RESEARCH INSTITUTE |
发明人 |
SORSCHER, ERIC J.;BENNETT, JR., LEONARD L.;PARKER, WILLIAM B. |
分类号 |
A61K38/45;A61K47/48;(IPC1-7):A61K48/00;C12N15/00 |
主分类号 |
A61K38/45 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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