| 摘要 |
PURPOSE:To obtain the subject gene, containing a benzene dioxygenase gene and cisbenzene glycol dehydrogenase gene, etc., and capable of exhibiting a specific restriction enzyme map and efficiently affording cisbenzene glycol from benzene. CONSTITUTION:Chromosomic DNA is separated from Pseudomonas.aeruginosa J1104 strain (FERM P-12180) having bezene-assimilating ability and digested with a restriction enzyme Xhol to afford the objective gene which is a DNA of about 7.2kb, containing a benzene dioxygenase gene, cisbenzene glycol dehydrogenase gene and catechol 2,3-oxygenase gene and having the number of cleavage with restriction enzymes as follows. PstI and SphI; 6, SalI; 5, EcoRI (E) and BamHI (B); 2, SmaI (Sm), BglII (Bg), SacI (Sa), XhoI (X) and ClaI (Cl); 1 and XbaI, HindIII and KpnI; 0 and further a restriction enzyme map expressed by the formula. |
