| 摘要 |
The invention provides a method of analysing and sequencing saccharide material comprising saccharide chains which contain more than three monosaccharide units to obtain sequence information. The method comprises the sequential steps of: (a) subjecting said saccharide material to partial depolymerisation by controlled treatment with a selective scission reagent to produce a mixed set of saccharide chains, intact chains and fragments of intact chains, having different lengths representative of the full spectrum of all possible lengths; (b) treating a sample of said mixed set of saccharide chains and chain fragments with said exoenzymes to an extent sufficient to obtain complete digestion and cleave susceptible linkages at the non-reducing end of all the saccharide chains and chain fragments and chain fragments and then (c) analysing said sample or samples to detect the various saccharide chain fragments generated by the cleavage treatments which are present therein, and obtaining, collectively from the results of said analysis, information enabling the monosaccharide sequence in the original saccharide material to be at least partially deduced; wherein substantially all of the saccharide chains and chain fragments produced by step (a) are labelled. |
