NOVEL ENDO-XYLOGALACTURONASE

摘要

Polypeptides possessing a novel activity, namely endo-xylogalacturonase activity, are disclosed. These polypeptides can degrade pectin found in plant extracts and plant materials, and in particular the "hairy" regions of pectin polymers. In particular, the polypeptides can cleave in a galacturonic acid polymer at internal glycocidic bonds. The novel enzyme XghA is disclosed, and its amino acid sequence and encoding DNA sequence given. This polypeptide was expressed in yeast cells and has been used to treat vegetable material, in particular soy and fruit juice in the preparation of edible foodstuffs.