发明名称 |
USE OF NUCLEAR MAGNETIC RESONANCE TO IDENTIFY LIGANDS TO TARGET BIOMOLECULES |
摘要 |
<p>A method for designing and forming a ligand which binds to a specific target molecule comprises the steps of: (a) identifying a first ligand moiety that binds to the target molecule using two-dimensional <15>N/<1>H NMR correlation spectroscopy; (b) identifying subsequent ligand moieties that bind to the target molecule using two-dimensional <15>N/<1>H NMR correlation spectroscopy; (c) forming a complex of the first and subsequent ligand moieties to the target molecule; (d) determining the three-dimensional structure of the complex and, thus, the spatial orientation of the first and subsequent ligand moieties on the target molecule; and (e) linking the first and subsequent ligand moieties to form a new ligand to maintain the spatial orientation of the ligand moieties. <IMAGE></p> |
申请公布号 |
EP0866967(B1) |
申请公布日期 |
2000.01.19 |
申请号 |
EP19960940448 |
申请日期 |
1996.11.13 |
申请人 |
ABBOTT LABORATORIES |
发明人 |
FESIK, STEPHEN, W.;HAJDUK, PHILIP, J. |
分类号 |
G01R33/465;C12Q1/00;G01N;G01N24/08;G01N33/15;G01N33/50;G01N33/53;G01N33/542;G01N33/566;G01N33/58;G01N33/68;(IPC1-7):G01N33/50 |
主分类号 |
G01R33/465 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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