Chimeric mice that express a human antibody

摘要

A process for constructing a chimeric non-human animal characterized by preparing microcells containing one or more foreign chromosomes or fragments thereof, and transferring the one or more foreign chromosomes or fragments thereof into pluripotent cells through fusion with the microcells; chimeric non-human animals which can be constructed by the above process and offspring thereof; tissues and cells originating in the above animals; methods for using the individuals, tissues and cells of the animals; pluripotent cells containing one or more foreign chromosomes or fragments thereof, a process for constructing these cells, and the use of these cells; pluripotent cells in which at least two intrinsic genes have been disrupted; a process for constructing the above cells by homologous recombination; etc.