发明名称 |
Identification of genes |
摘要 |
A method for identifying a microorganism having a reduced adaptation to a particular environment comprising the steps of: (1) providing a plurality of microorganisms each of which is independently mutated by the insertional inactivation of a gene with a nucleic acid comprising a unique marker sequence so that each mutant contains a different marker sequence, or clones of the said microorganism; (2) providing individually a stored sample of each mutant produced by step (1) and providing individually stored nucleic acid comprising the unique marker sequence from each individual mutant; (3) introducing a plurality of mutants produced by step (1) into the said particular environment and allowing those microorganisms which are able to do so to grow in the said environment; (4) retrieving microorganisms from the said environment or a selected part thereof and isolating the nucleic acid from the retrieved microorganisms; (5) comparing any marker sequences in the nucleic acid isolated in step (4) to the unique marker sequence of each individual mutant stored as in step (2); and (6) selecting an individual mutant which does not contain any of the marker sequences as isolated in step (4).
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申请公布号 |
US6015669(A) |
申请公布日期 |
2000.01.18 |
申请号 |
US19970871355 |
申请日期 |
1997.06.09 |
申请人 |
IMPERIAL COLLEGE INNOVATIONS LIMITED |
发明人 |
HOLDEN, DAVID WILLIAM |
分类号 |
C12N15/09;A61K31/7088;A61K38/00;A61K39/00;A61K39/02;A61K39/112;A61K48/00;A61P31/04;A61P31/10;C07H21/02;C07K14/21;C07K14/255;C07K14/315;C07K14/38;C12N1/10;C12N1/15;C12N1/21;C12N15/00;C12N15/10;C12N15/11;C12N15/31;C12Q1/04;C12Q1/68;G01N33/53;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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