发明名称 |
CDNS-KLONIERUNGSVERFAHREN FÜR REZEPTORTYROSINKINASEZIELPROTEIN UND HGRB- PROTEINE |
摘要 |
<p>A novel expression cloning method is provided for the detection, identification and purification of target proteins capable of binding at least to a tryosine-phosphorylated domain of a eukaryotic tyrosine kinase using novel peptide probes comprising an amino acid sequence substantially corresponding to a portion of a tyrosine-phosphorylated domain of a tyrosine kinase. The probe has at least one phosphorlated tyrosine residue and may be detectably labeled. Also disclosed is a method for preparing the probes a method for mapping to a chormosome a gene encoding a protein capable of binding to tyrosine-phosphorylated domains of tyrosine kinases, and a method for purifiying such a protein with the probe. Non-limiting examples of novel proteins discovered using the above cloning method include GRB-1, GRB-2, GRB-3, GRB-4 and GRB-7, as well as nucleic acid encoding these proteins, and methods for detecting these proteins are also provided.</p> |
申请公布号 |
AT187772(T) |
申请公布日期 |
2000.01.15 |
申请号 |
AT19920904444T |
申请日期 |
1992.01.17 |
申请人 |
NEW YORK UNIVERSITY |
发明人 |
SCHLESSINGER, JOSEPH;SKOLNIK, EDWARD, Y.;MARGOLIS, BENJAMIN, L. |
分类号 |
C12N15/09;A61K38/00;C07K1/22;C07K14/47;C07K14/475;C07K14/705;C07K14/71;C07K14/715;C07K16/28;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/12;C12P21/02;C12P21/08;C12Q1/02;C12Q1/68;C12R1/01;G01N33/53;(IPC1-7):C12N15/12;G01N33/68;C12N15/62 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|