发明名称 |
NOVEL NONVIRAL GENE CARRIER AND PREPARATION THEREOF |
摘要 |
PURPOSE: A method of delivering genes into various liver cancer cells using virus-like particles (VLP) is provided to improve delivering efficiency by binding a gene to VLP then treating with polyoxyethylene-polyoxypropylene block copolymer. CONSTITUTION: A method comprises the following steps of: producing VLP from recombinant protein obtained by infecting Papillomavirus capsid antigen or other virus capsid antigen into insect cells; producing VLP-PL conjugate by conjugating polylysine with VLP; obtaining VLP-PL-pCMV beta-gal plasmid complex, VLP-PL-pSV2CAT plasmid complex or VLP-PL-pCMV-p53 plasmid complex by linking plasmid pCMV beta-gal, pSV2CAT, pCMV-p53, respectively; treating the complex with polyoxyethylene-polyoxypropylene block copolymer before transfecting into liver cancer cell line.
|
申请公布号 |
KR20000000844(A) |
申请公布日期 |
2000.01.15 |
申请号 |
KR19980020709 |
申请日期 |
1998.06.03 |
申请人 |
KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY |
发明人 |
PARK, SUN HUI;JO, JEONG WON;KANG, BYEONG TAE;JO, YEONG SIK;JEONG, MI KYEONG;KIM, YEON SU |
分类号 |
C12N15/86;C12N15/00;(IPC1-7):C12N15/86 |
主分类号 |
C12N15/86 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|