NOVEL GENE ENCODING TREHALOSE BIOSYNTHETIC ENZYME AND PRODUCTION METHOD OF TREHALOSE THEREBY

摘要

PURPOSE: Genes encoding Brevibacterium maltooligosyltrehalose synthease (BvMTase) and Brevibacterium maltooligosyl trehalose trehalohydrolase (BvMTHase) are isolated to obtain trehalose in large quantities which is used as food additives or food preservatives. CONSTITUTION: Trehalose is produced by following steps of: isolating gene encoding BvMTase and BvMTHase from Brevibacterium helvolum (ATCC 11822); cloning the gene into an expression vector which consists of 4,515 bp and encodes two enzyme proteins; massively expressing the recombinant proteins (enzymes) in E. coli; purifying the enzymes; and producing trehalose from maltooligosaccharide or starch by the enzymes. The structural gene of BvMTase gene consists of 2,328 bp; BvMTase consists of 776 amino acids. and has a molecular weight of about 85.8 kDa; the structural gene of BvMVHase consists of 1,767 bp; BvMTHase consists of 589 amino acids. and has a molecular weight of about 64.2 kDa.