发明名称 |
MULTIPLEX POLYMERASE CHAIN REACTION-BASED METHOD FOR QUICK-DETECTION OF HOSPITAL COLON BACILLUS AND PCR PRIMER |
摘要 |
PURPOSE: A multiplex polymerase chain reaction-based method is provided to quickly detect E. coli gene producing heat-labile enterotoxin and heat-stable enterotoxin, which cause diarrhea of live stock. CONSTITUTION: ST(heat-stable enterotoxin) I primer is synthesized to be based on the nucleotide sequence of ST I a(p) gene separated from a pig. A PCR method for detecting gene uses a pair of primers, a forward primer and a reverse primer. Heat-labile enterotoxin gene and heat-stable enterotoxin gene, which are separated from E.coli, are detected simultaneously by performing PCR with mixing PCR primers therefor.
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申请公布号 |
KR20000003286(A) |
申请公布日期 |
2000.01.15 |
申请号 |
KR19980024503 |
申请日期 |
1998.06.27 |
申请人 |
REPUBLIC OF KOREA(MANAGEMENT : MINISTRY OF AGRICULTURE AND FORESTRY, NATIONAL VETERINARY RESEARCH INSTITUTE) |
发明人 |
WOO, SEUNG RYONG;LIM, SOOK KYUNG;LEE, HEE SOO;KIM, JONG MAN;KIM, JONG YEOM |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
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