POLYNUCLEOTIDE ASSAY APPARATUS AND POLYNUCLEOTIDE ASSAY METHOD

摘要

A polynucleotide assay method with the use of a polynucleotide detection cell provided with a first electrode (111) containing DNA probes (13, 14, 15, 16) fixed to respective different compartments (3, 4, 5, 6) and second electrodes (113-1, 113-2) opposite to the first electrode. The method comprises capturing a target polynucleotide by complementary strand binding between each DNA probe fixed to each compartment and the target polynucleotide, conducting an elongation reaction by using a base (dNTP) labeled with electrochemiluminescence to elongate the DNA probe bonded by complementary strand binding, and detecting electrochemiluminescence created by applying a voltage across the first electrode and the second electrodes to detect whether or not an elongated chain produced by the elongation reaction is present. The DNA detection cell having a simple constitution and an assay apparatus using the same serve to quickly detect a hybrid of a target DNA fragment with a DNA probe and to assay a large amount of probes in a short time.