Breast tumor cells for study of nonionizing radiation effects

摘要

The cell line EMT-6 are transformed with the chromosomal insertion of the plasmid pSV2neoNR101, ATCC No. 69617. The transformed cells, EMT-6/pSV2neoNR101, produce diazoluminomelanin (DALM) intracellularly when provided with nitrate, luminol and 3-amino-L-tyrosinexHCl (3AT). The modified cells can be used to study mechanisms for radiofrequency and light radiation interactions with breast tumor cells in vitro and in mice. The effects of drugs, hormones, and cytokines that affect the expression of nitric oxide synthase and its activity can also be studied to understand the effects of these materials on breast tumor cells.