摘要 |
PROBLEM TO BE SOLVED: To provide a method for efficiently growing the F1 species of genus Eustoma plant in a short time by culturing ovules under specific conditions using false fertilization on the pistils of male sterility type genus Eustoma plant or the like, to create the genetically pure genus Eustoma plant. SOLUTION: This method comprises steps of (A) pollination of the ovules of a male sterility type or castrated genus Eustoma plant with the pollen irradiated with ionizing radiation of 50 KR or more, preferably 50 to 200 KR to deactivate its cell nuclei, (B) picking up the ovules 2 to 10 weeks, preferably 3 to 4 weeks after the pollination, and (C) culturing and growing the ovules in a medium incorporated with, e.g. coconut milk, to create the genetically pure genus Eustoma plant.
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