| 摘要 |
Modified GnRH receptors (GnRH-Rs), including human GnRH receptors, are described, together with polynucleotides encoding therefor. The modified GnRH-Rs may be expressed in stable cell lines at significantly higher levels than the equivalent wild-type receptor, whilst the binding characteristics of the receptor remain essentially unchanged. Modifications to human GnRH-R which increase expression include deletion of lysine 191 and addition of a catfish C-terminal tail, optionally also including a C-terminal multi-histidine tag. |
