METHOD FOR CREATING POLYNUCLEOTIDE AND POLYPEPTIDE SEQUENCES
摘要
The invention provides methods for evolving a polynucleotide toward acquisition of a desired property. Such methods entail incubating a population of parental polynucleotide variants under conditions to generate annealed polynucleotides comprising heteroduplexes. The heteroduplexes are then exposed to a cellular DNA repair system to convert the heteroduplexes to parental polynucleotide variants or recombined polynucleotide variants. The resulting polynucleotides are then screened or selected for the desired property.
申请公布号
WO9929902(A9)
申请公布日期
2000.01.06
申请号
WO1998US25698
申请日期
1998.12.04
申请人
CALIFORNIA INSTITUTE OF TECHNOLOGY;ARNOLD, FRANCES;SHAO, ZHIXIN;VOLKOV, ALEXANDER