| 摘要 |
This invention provides means of measuring oxidative response of cells witho ut recourse to preparation of cell culture. The process consists of: 1) prepari ng suspensions of cells from a living host in isotonic solutions, 2) preparing samples of test materials in isotonic solution containing tagged choline, 3) adding the cells suspension prepared in step 1 to the samples prepared in st ep 2, 4) incubating the product of step 3 with shaking for 2-90 minutes, 5) extracting and drying the lipid phase from the product of step 4, and 6) measuring the choline that has been incorporated onto PC in the product of step 5. An increase in incorporation of choline into PC in the short term indicates oxidative stress of free radical induced damage. Because the metho d of the invention using the cell isolates does not require the expense of cel l culture with concomitant expense and possibility of cell change, it is particularly useful for clinical evaluation. Furthermore, the ability to use erythrocytes for study of cellular response to oxidative stress makes it possible to test individual responses of patient using part of the blood sample drawn routinely for laboratory use.
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