TAGGING OF RNA AMPLICONS GENERATED BY TRANSCRIPTION-BASED AMPLIFICATION

摘要

The present invention concerns a method for tagging the reaction products of transcription-based amplification procedures, and a kit for detecting such tagged nucleic acid amplified in a transcription-based amplification assay. The method is characterised in that RNA amplicons are generated by transcription-based amplification by introducing transcribable non-target related sequence elements in one or both primers used for transcription-based amplification which are transcribed into the RNA amplicons. In particular, the extra sequence elements thus introduced into the amplicons, can be used for the detection of the amplicons, for specific selection of the amplicons, to artificially extend the length of amplicons or to tag polypeptides translated from RNA amplicons generated by transcription-based amplification.