Recombinant production of toxoplasma sag1 antigen

摘要

The invention provides a method for the production of the toxoplasma antigen SAG1 or a fragment thereof, which comprises: (a) constructing a plasmid comprising DNA encoding SAG1 or a fragment thereof; (b) transforming a P. pastoris host cell with the said plasmid; and culturing the host such that the DNA encoding SAG1 or a fragment thereof is expressed. The invention further provides truncated SAG1 proteins in which the anchor region is absent, particularly truncated SAG1 comprising amino acid residues (48-307) of SAG1, and vaccine compositions comprising the SAG1.