发明名称 |
Cell ablation using trans-splicing ribozymes |
摘要 |
The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described.
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申请公布号 |
US6010904(A) |
申请公布日期 |
2000.01.04 |
申请号 |
US19950482658 |
申请日期 |
1995.06.07 |
申请人 |
THE GENERAL HOSPITAL CORPORATION;PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
HASELOFF, JAMES;BRAND, ANDREA;PERRIMON, NORBERT;GOODMAN, HOWARD M. |
分类号 |
C12N15/113;(IPC1-7):C12N15/85;C12N15/63;C07H21/04 |
主分类号 |
C12N15/113 |
代理机构 |
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