发明名称 |
Methods of preparing and recovering proteins |
摘要 |
There is disclosed the preparation and recovery of proteins, in particular of enzymes, by controlled proteolytic cleavage of pro-proteins, in particular pro-enzymes, in a single method step, wherein a pro-protein-containing solution is contacted with a protease and with a solid carrier which has a higher affinity to the protein than to the pro-protein or to the functionally inactive degradation products thereof, the pro-protein being proteolytically cleaved to said protein, and the protein being selectively separated by absorption on the solid carrier.
|
申请公布号 |
US6010844(A) |
申请公布日期 |
2000.01.04 |
申请号 |
US19960752892 |
申请日期 |
1996.11.20 |
申请人 |
BAXTER AKTIENGESELLSCHAFT |
发明人 |
FISCHER, BERNHARD;MITTERER, ARTUR;DORNER, FRIEDRICH;EIBL, JOHANN |
分类号 |
C12P21/00;A61K38/43;A61K38/46;A61K38/55;A61P7/00;A61P7/02;C07K1/04;C07K1/22;C07K14/745;C07K19/00;C12N9/50;C12N9/64;C12N9/74;C12P21/06;(IPC1-7):C12Q1/00;G01N33/53;A61K38/00;C07K1/00 |
主分类号 |
C12P21/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|