发明名称 |
PCR PRIMER CONSTRUCTS FOR USE IN AN INTEGRATED SIGNALLING SYSTEM |
摘要 |
A method for the detection of a target nucleic acid, which method comprises contacting template nucleic acid from a sample with (i) a signalling system and (ii) a tailed nucleic acid primer having a template binding region and t he tail comprising a linker and a target binding region, in the presence of appropriate nucleoside triphosphates and an agent for polymerisation thereof , under conditions such that the template binding region of the primer will hybridise to a complementary sequence in the template nucleic acid and be extended to form a primer extension product, separating any such product fro m the template whereupon the target binding region in the tail of the primer will hybridise to a sequence in the primer extension product corresponding t o the target nucleic acid, and wherein any such target specific hybridisation causes a detectable change in the signalling system such that the presence o r absence of the target nucleic acid in the sample is detected by reference to the presence or absence of a detectable change in the signalling system.</SD OAB>
|
申请公布号 |
CA2377508(A1) |
申请公布日期 |
1999.12.23 |
申请号 |
CA19982377508 |
申请日期 |
1998.11.25 |
申请人 |
DXS LIMITED |
发明人 |
GIBSON, NEIL JAMES;WHITCOMBE, DAVID MARK;THEAKER, JANE;LITTLE, STEPHEN |
分类号 |
G01N33/50;C12N15/09;C12Q1/68;G01N33/58;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/50 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|