发明名称 |
DNS-ABHÄNGIGE RNS-POLYMERASE-TRANSKRIPTE ALS REPORTERMOLEKÜLE ZUR SIGNALVERSTÄRKUNG IN NUKLEINSÄURE-HYBRIDISIERUNGSUNTERSUCHUNGEN |
摘要 |
A polydeoxynucleotide construct is disclosed for use, in conjunction with a DNA-dependent RNA polymerase, as a signal amplifier in nucleic acid hybridization assays. The construct contains a recognition sequence for a target oligonucleotide, a promoter sequence for a DNA-dependent RNA polymerase, and a polymerase template. A method of use for this construct in hybridization assays is also disclosed. The method involves formation of a hybridization complex comprising the construct and the target sequence; addition of a polymerase which is specific for the promoter in the construct; and quantification of the resulting RNA transcripts. |
申请公布号 |
DE69131596(T2) |
申请公布日期 |
1999.12.23 |
申请号 |
DE1991631596T |
申请日期 |
1991.01.10 |
申请人 |
CHIRON DIAGNOSTICS CORP., EAST WALPOLE |
发明人 |
URDEA, MICHAEL |
分类号 |
C12N15/09;C07H19/06;C07H21/00;C12N15/10;C12Q1/68;C12Q1/70;G01N33/532;G01N33/569;G01N33/576 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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