Thermostabilization, osmoprotection, and protection against desiccation of enzymes, cell components and cells by di-glycerol-phosphate

摘要

The utilization of di-glycerol-phosphate as a stabiliser of enzymes and other cell components or cells, namely against heat, freezing, desiccation, lyophilization or repeated usage, is disclosed. Examples with rubredoxin, lactate dehydrogenase and alcohol dehydrogenase show that this compound is a better protecting agent than glycerol. This solute is produced by organic chemical synthesis readily and efficiently in three steps from commercially available glycerol in its racemic forms. The optically pure forms can be synthesised in four steps from commercially available D-mannitol. Di-glycerol-phosphate is obtained in a highly pure state without the necessity of complicated and tedious purification procedures.