| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for the comparative analysis of nucleic acid sequences through making use of the fragment analysis of fragmented sample nucleic acid. SOLUTION: This method for the comparative analysis of a nucleic acid sequence comprises the following procedure: a wild type-derived sample DNA 1 and a variant type-derived sample DNA 2 are fragmented with restriction enzymes, respectively, to prepare two kinds of 1st fragment group 4 and 5, which are then mixed with each other to effect complete modification followed by complementary strand formation again, thereby affording a 2nd fragment group 8 made up of completely complementary strands 9, 10, and complementary strands 11 formed between variant type-derived fragments and wild type-derived fragments; subsequently, only the single strand 14 portion of the variant part 3 of the fragments 11 is specifically cleaved with an enzyme to afford a 3rd fragment group 15; the fragment analysis of the fragments of the 3rd fragment group and that of the fragments formed of the wild type-derived fragments alone are then performed and the electrophoretic patterns of both kinds of fragment are compared with each other; thereby the presence/absence of the variation in the variant type sample DNA 2 can be confirmed, and the base sequence can be determined by selectively amplifying only the fragment containing the variant part 3. |
