摘要 |
A process is disclosed for preparing a protein by a eukaryote transformed by multicopy integration of an expression vector into the genome of a yeast, such as Saccharomyces, Hansenula, and Kluyveromyces, or of a mould such as Aspergillus, Rhizopus and Trichoderma, the expression vector containing both an "expressible gene" encoding the protein and a so-called "deficient selection marker needed for the growth of the yeast or mould in a specific medium", such as the LEU2d, TRP1d or URA3d gene, in combination with a ribosomal DNA sequence, resulting in stable high copy integration of 100-300 copies per cell. This multicopy integration results in an increased production of the desired protein, which can be guar alpha -galactosidase, an oxidase or a hydrolytic enzyme such as a lipase.
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申请人 |
UNILEVER PATENT HOLDINGS, B.V. |
发明人 |
GIUSEPPIN, MARCO F.;LOPES, MARIA T. S.;PLANTA, ROELF J.;VERBAKEL, JOHANNES M. A.;VERRIPS, CORNELIS T. |