ALPHA-INTERFERON MANUFACTURING PROCESS USING IMMUNOADSORPTION AND VIRUS REMOVAL FILTRATION

摘要

The present invention concerns a method of manufacturing alpha -interferon product from crude interferon preparations obtained from human or animal cell culture or from transgenic animals. The method comprises contacting a solvent/detergent treated composition with at least two monoclonal mouse IgG antibodies having complementary subtype specificities in an immunoadsorption step. The alpha -interferon subtypes bound by the monoclonal antibodies are eluted and the eluate is purified and filtered on a virus removal filter. The method according to the invention effectively removes all types of impurities present in crude interferon products, and gives rise to a highly purified and virus-safe multicomponent interferon which contains major alpha -interferon subtypes.