| 摘要 |
<p>1,224,958. Ribonucleic acid. MERCK & CO. Inc. 22 Sept., 1969, No. 46577/69. Heading C3H. A process for obtaining double-stranded RNA comprises adding Escherichia coli to a nutrient culture medium and permitting growth to occur, adding Mu-9 mutant to infect the growing E. coli, then adding an antibiotic to inhibit the growth of the E. coli and thereby obtain an increased productivity of DS-RNA relative to the E. coli cells. Suitable media include tryptone and corn-steep liquor. The antibiotic may be streptomycin, oxytetracycline or chloramphenicol added in an amount of 5 to 200 Ág per ml. of culture at 45 to 120 minutes after infection.</p> |
