发明名称 |
DNA polymerase having 3'-intrinsic editing activity |
摘要 |
The invention is directed to the use of RNA or DNA polymerases having 3'-intrinsic editing activity (3'-IEA) in the presence or absence of a deactivating agent to remove a protecting group from the 3'-position of oligo- polyribo- or deoxyribonnucleotides. The use is connected with the incorporation of dNTPs into DNA templates in order to determine the concentration and/or sequence of said templates. In particular the use is concerned with an improved non gel-based sequencing method.
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申请公布号 |
US6001566(A) |
申请公布日期 |
1999.12.14 |
申请号 |
US19960655777 |
申请日期 |
1996.05.31 |
申请人 |
ROCHE DIAGNOSTICS GMBH |
发明人 |
CANARD, BRUNO;SAGNER, GREGOR |
分类号 |
C12N15/09;C07H21/02;C07H21/04;C12N9/12;C12Q1/48;C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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