Adenovirus and methods of use thereof

摘要

PCT No. PCT/US95/14017 Sec. 371 Date Aug. 25, 1997 Sec. 102(e) Date Aug. 25, 1997 PCT Filed Oct. 27, 1995 PCT Pub. No. WO96/13597 PCT Pub. Date May 9, 1996A recombinant adenovirus and a method for producing the virus are provided which utilize a recombinant shuttle vector comprising adenovirus DNA sequence for the 5' and 3' cis-elements necessary for replication and virion encapsidation in the absence of sequence encoding viral genes and a selected minigene linked thereto, and a helper adenovirus comprising sufficient adenovirus gene sequences necessary for a productive viral infection. Desirably, the helper gene is crippled by modifications to its 5' packaging sequences, which facilitates purification of the viral particle from the helper virus.