发明名称 |
Cyanine dye activating group with improved coupling selectivity |
摘要 |
Activating groups for cyanine dyes used to label chain terminators in nucleotide sequencing, based on N-hydroxyphthalimide, are disclosed. From these activating groups, activated dyes of the present Invention are prepared which react with the derivitized nucleotide chain terminators to give a labeled chain terminator of the present Invention. The activating groups of the present Invention allow the dye-chain terminator reaction to occur at a much higher yield and with much greater selectivity for the mono-substituted product, compared with the prior art.
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申请公布号 |
US6002003(A) |
申请公布日期 |
1999.12.14 |
申请号 |
US19980059900 |
申请日期 |
1998.04.14 |
申请人 |
BECKMAN INSTRUMENTS, INC. |
发明人 |
SHEN, GENE G.-Y.;DOBASHI, THOMAS S. |
分类号 |
C07D209/08;C07D209/60;C07H19/10;C07H19/20;C09B23/08;(IPC1-7):C07F9/09;C07F9/141;C07D207/408;C07D487/04 |
主分类号 |
C07D209/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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