| 摘要 |
<p>The invention relates to a process for obtaining through genetic engineering plants with an improved capacity to synthesize, accumulate and exudate organic acids. More specifically, it relates to the generation of transgenic plants which have an improved capacity for the production and excretion of organic acids, providing them with an improved capacity to absorb natural nutrients for the soil or added nutrients such as fertilizers. Said plants have also an incremented capacity to tolerate the presence in the soil of certain toxic compounds such as aluminium. The transformation process implies the introduction of genes which increase the capacity of the plant to produce organic acids and comprises the following steps: a) preparation of a recombinant molecule comprising the coding sequence for an enzyme which produces organic acids, functionally ligated to a promoter sequence which is active in vegetable cells and a terminator of the functional transcription in vegetable cells; b) transformation of vegetable cells with said construction; c) regeneration of transgenic plants from transformed cells.</p> |
