| 摘要 |
The invention details compositions of DNA sequence with the ability to confer a given relative binding affinity to any DNA ligand whose binding site is placed adjacent to the DNA sequence composition. Further, this invention features a method of creating and thereby systematically sampling polynucleotides of any given length sequence space for polynucleotide sequences given the nucleotides A, C, G, and T/U. This invention also features a method of grading the sequences created into a range of relative binding affinities from high binding affinity to low binding affinity, with respect to conferring a given relative binding affinity of any DNA ligand to its binding site placed adjacent to a polynucleotide sequence based on its nucleotide composition. In another aspect this invention features an algorithm for grouping homologous polynucleotide sequences into families related solely by their nucleotide composition. This invention also relates to methods for utilizing a member of a family to allow for the prediction of a region of any polynucleotide sequence or protein which is susceptible to mutation, for modulating the mutation rate of any polynucleotide sequence or protein, for altering the relative expression level of any gene, and the like. |
