DNA detection by a strand reassociation complex

摘要

A method for selective detection of nucleic acids comprises amplification of the nucleic acid with the help of labeled primers and detection with a trap probe. A method for selective detection of nucleic acids comprises: amplification of a nucleic acid or a part of it with the help of two primers, one of which hybridizes to the assigned nucleic acid and the other to its complementary strand and at least one of which is detectably marked, which in a reaction mixture these primers form an extension product; binding of a trap probe to the extension product to form a hybridization complex containing the trap probe and at least the extension product; separation of the trap probe bound extension product from non bound parts of the reaction mixture; and determining the bound trap probe by the detectable marker, characterized in that the trap probe was chosen so that it could bind to a strand of the extension product and also can hybridize with the marked primers that are forming the extension product. Independent claims are also included for: a method to selectively detect a specific sequence in one or more nucleic acids; and a reagent kit for detection of nucleic acids.