Rhodamine derivates and their use in diagnostic systems

摘要

Rhodamine derivatives (I) and (II), having specific substituents in the phenyl ring, are new. Rhodamine derivatives of formulae (I) and (II) and their activated derivatives and conjugates are new. The novel feature of the structure is the nature of the substituents A1-A3, B1 and B2. Dotted line = single or double bond; X1-X16 = halo, SO3H, H or 1-20C alkyl (optionally substituted by one or more halo or SO3H groups); R1, R2 = H, 1-20C alkyl, polyoxyhydrocarbyl, phenyl or phenyl-(1-3C) alkyl (phenyl and/or alkyl groups are optionally substituted by at least one OH, halo, SO3H, NH2, COOH or (1-4C) alkoxycarbonyl); A1-A3 = H, CN, halo or SO3H; B1 = halo, CN or H; B2 = H, amido, halo or 1-20C alkyl; provided that at least R1 contains at least one activatable group and R2 is optionally linked to X4 by a bridge containing 0-2 atoms.