| 摘要 |
The present invention relates to a process for quantifying entities, comprising the steps of (a) mixing an effective detection amount of a (proto-) porphyrin represented by formula (I) wherein R1 independently represents a radical selected from the group consisting of -CH(OH)-CH3, -CH=CH2, -CH2-CH3, -H, -COCH3, -CHO, -CH(OH)-CH2OH, and -CH=CHO2H; R2 independently is selected from C1-3 alkyl, and preferably is methyl, R3 independently represents an aryl or aralkyl group, and preferably is phenyl; and M is a metal selected from Fe, Co, Ga, Sn, Zn, Cr, Mg, Ni, Ge, and Cu, with a sample suspected to contain entities to be detected; (b) collecting the (proto)porphyrin containing complexes formed; and (c) detecting and quantifying the collected complexes. Further, the invention relates to a chemiluminescent composition comprising a compound defined in formula I.
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