发明名称 |
L-isoleucine-producing bacterium and method for preparing L-isoleucine through fermentation |
摘要 |
An L-isoleucine-producing bacterium belonging to the genus Escherichia which carries a thrABC operon which comprises a thrA gene coding for aspartokinase I-homoserine dehydrogenase I substantially released from the inhibition by L-threonine and an ilvGMEDA operon which comprises an ilvA gene coding for threonine deaminase substantially released from the inhibition by L-isoleucine and whose region required for attenuation is removed; and an L-isoleucine-producing bacterium belonging to the genus Escherichia carrying the thrABC operon, an lysC gene coding for aspartokinase III substantially released from the inhibition by L-lysine, and the ilvGMEDA operon. The bacteria permit biosynthesis of a sufficient amount of L-isoleucine.
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申请公布号 |
US5998178(A) |
申请公布日期 |
1999.12.07 |
申请号 |
US19950452075 |
申请日期 |
1995.05.26 |
申请人 |
AJINOMOTO CO., LTD. |
发明人 |
HASHIGUCHI, KEN-ICHI;KISHINO, HIROKO;TSUJIMOTO, NOBUHARU;MATSUI, HIROSHI |
分类号 |
C12N1/21;C12N15/52;C12P13/06;(IPC1-7):C12P13/06 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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