| 摘要 |
The present invention relates to methods for producing a polypeptide in enhanced amounts, comprising (a) cultivating a mutant of a parent filamentous fungal cell in a nutrient medium suitable for production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a modification of one or more second nucleic acid sequences encoding DDC2 and/or DDC3 polypeptides, or homologues thereof, and (ii) the mutant cell produces more of the polypeptide than the parent cell when cultured under the same conditions; and (b) recovering the polypeptide from the nutrient medium of the mutant cell. The present invention also relates to the second nucleic acid sequences, polypeptides encoded by the second nucleic acid sequences, and nucleic acid constructs, recombinant expression vectors, and host cells comprising the sequences. The present invention further relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. |
