发明名称 |
METHOD FOR THE ISOLATION OF CCC PLASMID DNA |
摘要 |
The present invention relates to the production of biomass for the isolation of ccc plasmid DNA comprising culturing a bacterial transformant in a bioreactor containing an antibiotic-free batch medium under batch-conditions and, at the end of the batch phase, feeding under feed-back conditions the portion of a feed-back medium after the rise of DO above a threshold-set point. Said feed-back medium comprises besides a carbon source a magnesium salt, preferably in concentrations above 20 mM. Preferably, the bacterial transformant is harvested after the end of the culture and frozen or freeze-dried. Also preferred is that ccc plasmid DNA is, optionally directly, isolated after harvesting the bacteria. |
申请公布号 |
WO9961633(A2) |
申请公布日期 |
1999.12.02 |
申请号 |
WO1999EP03522 |
申请日期 |
1999.05.21 |
申请人 |
QIAGEN GMBH;SCHMIDT, TORSTEN;FRIEHS, KARL;FLASCHEL, ERWIN;SCHLEEF, MARTIN |
发明人 |
SCHMIDT, TORSTEN;FRIEHS, KARL;FLASCHEL, ERWIN;SCHLEEF, MARTIN |
分类号 |
C12N15/09;C12N1/20;C12N1/21;C12N15/10;C12N15/70;C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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