| 摘要 |
<p>The present invention provides for a method of identifying a ligand for a receptor comprising contacting a cell expressing a cell surface FcεR1 molecule with a chimeric polypeptide molecule comprising an extracellular ligand binding domain of a receptor and an IgE constant or Fc region, under conditions whereby the chimeric polypeptide molecule binds to the FcεR1 molecule to form a complex; contacting the cell bearing the complex with a ligand; and detecting or measuring ligand binding to the complex. The invention further provides for chimeric polypeptide molecules, the nucleic acids encoding the chimeric polypeptide molecules, and cell lines expressing the chimeric polypeptide molecules.</p> |
