摘要 |
<p>The present invention includes methods of performing ex vivo expansion of gene-modified hematopoietic stem cells which are useful for many applications involving bone marrow transplantation and ex vivo gene therapy. The present invention further includes the gene-modified hematopoietic stem cells that are used and produced by such methods. Such gene-modified hematopoietic stem cells can also contain a second heterologous gene. In addition, the present invention also includes methods of engrafting the gene-modified hematopoieteic stem cells of the present invention into animals, including for ex vivo gene therapy and for reconstitution of hematopoieteic cells in ablated mammals.</p> |