BIFUNCTIONAL MOLECULES FOR BINDING AND REGULATING E-SELECTINS AND METHODS DETECTING SAME

摘要

The present invention provides novel bifunctional compounds for regulation of cellular proliferation. These bifunctional compounds include a cell-adhesion oligosaccharide attached to a linker group by the reducing end of the cell-adhesion oligosaccharide or by a primary hydroxyl group and said linker group also attached to a nucleoside cyclic-3'-5' monophosphate or analogue through a heterocyclic base. Specific oligosaccharide and nucleoside cyclic-3'-5' monophosphate and linkers are also shown. The bifunctional compound can also be used to regulate cell proliferation by contacting a therapeutic affect amount of the bifunctional compound with a selectin. There is also a method for detecting which compounds or other adhesion molecules have agonistic or antagonistic activity to cell proliferation by contacting the test compound with a selectin in a cell culture and measuring the growth of the cells in the cell culture, wherein a compound with agonistic activity will show increased cell growth or adhesion over normal, and a compound with antagonistic activity will show decreased cell growth or adhesion over normal.