5'-MODIFIED NUCLEOTIDES AND THE APPLICATION THEREOF IN MOLECULAR BIOLOGY AND MEDICINE

摘要

The invention relates to 5'-modified nucleotides and nucleic acids containing said nucleotides. The invention also relates to methods for integrating 5'-modified nucleotides in nucleic acids and subsequent localized splitting of the nucleic acids on the 5'-modified monomer structural elements. The inventive methods can be used in nucleic acid sequencing, in the creation of nucleic acid libraries, in the detection of mutations, in the production of carrier-bound nucleic acids and for pharmaceutical purposes.