METHOD FOR CHROMATOGRAPHIC SEPARATION

摘要

At least 2 different packings differing in the resolution of components contained in a starting fluid material such as a starting solution material and desired to be separated are used in a coexistent state to adjust the resolution of the components. The starting fluid material is flowed through the resultant packing bed to effect chromatographic separation, whereby high-purity fractions enriched with respective components can be obtained at high recoveries with a small amount of desorbent such as eluent. In a chromatographic separator, at least 2 packings comprising a first packing high in the resolution of a component having a medium affinity (affinity for packing) and a weak-affinity component and a second packing high in the resolution of a strong-affinity component and the medium-affinity component in a coexistent state are used as chromatographic packing in contact with a fluid containing the medium-affinity component to adjust the resolution of every two of at least 3 components, whereby at least 3 fractions can be obtained. Examples of the procedure of preparing a coexistent state of, e.g., 2 packings include a method wherein both are mixed and then packed, a method wherein both are packed in the stratified form of at least 2 layers, and a method wherein a certain packing bed unit(s) is packed with a first packing alone and the other packing bed unit(s) is packed with a second packing alone. An ion exchange resin is preferably used as chromatographic packing to simply provide at least 2 packings by changing the ionic form thereof. <IMAGE>